2019-02-19 19:04:542018-12-07 18:04:34

description

malate-H⁺/Na⁺-lactate antiporter

malate-H+/Na+-lactate antiporter

geneLength

1407

1404

product

malate-H⁺/Na⁺-lactate antiporter

malate-H+/Na+-lactate antiporter

Gene

Coordinates

2,452,800 2,454,206

2,452,800 → 2,454,206

Biological materials

Mutant

MGNA-C411 (yqkI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2409 NBRP B. subtilis, Japan]

GP1460 (''mleN''::''spc''), available in [SW|Stlke] lab

BKE23560 ([[gene|mleN]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE23560 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAAAGAAGTCCCCCTTCAT, downstream forward: _UP4_GGCTAATAGGGGAGGATCAC

BKK23560 ([[gene|mleN]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK23560 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAAAGAAGTCCCCCTTCAT, downstream forward: _UP4_GGCTAATAGGGGAGGATCAC

MGNA-C411 (yqkI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2409 NBRP B. subtilis, Japan]

GP1460 (''mleN''::''spc''), available in [SW|Stülke] lab

BKE23560 (Δ[[gene|mleN]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE23560 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAAAGAAGTCCCCCTTCAT, downstream forward: _UP4_GGCTAATAGGGGAGGATCAC

BKK23560 (Δ[[gene|mleN]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK23560 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAAAGAAGTCCCCCTTCAT, downstream forward: _UP4_GGCTAATAGGGGAGGATCAC

Biological materials

lacZ fusion

pGP388 (in [[protein|pAC7]]), available in [SW|Stlke] lab

pGP388 (in [[protein|pAC7]]), available in [SW|Stülke] lab